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A novel method for quantifying cell migration through tissue engineering scaffolds: evaluation of modified collagen matrices


Reference:

Bodamyali, T., Jarman-Smith, M., Stevens, C. and Chaudhuri, J., 2002. A novel method for quantifying cell migration through tissue engineering scaffolds: evaluation of modified collagen matrices. Biotechnology Letters, 24 (18), pp. 1491-1497.

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Abstract

A novel method to quantify cell migration through potential tissue engineering 3-d scaffolds is described. The migration assay uses a dot-blotting app. into which the tissue engineering matrix is placed on top of a nitrocellulose membrane. This assay was used to evaluate human dermal fibroblast migration through four porcine collagen matrixes with varying pore diams. and pitch lengths. Fibroblasts were placed on the matrix surface, at between 1 *103-3 * 103 cells mm-2, and left for 18 h to allow migration. The nitrocellulose membrane was stained with haematoxylin, the membrane digitised and the pixel intensity of the stained cells quantified. We showed that for all matrix variants, migration was more effective with a higher initial seeding d. The application of varying initial cell densities resulted in the greatest extent of cell migration through the matrix variant with pores of 30 micro m diam. and 400 micro m pitch length (i.e. 10.3% migration at 1 *103 cells mm-2). This method was coupled with confocal microscopy to evaluate the depth of cell migration within the matrix. At a depth of 20 micro m cell nos. were similar to those on the matrix surface: at a depth of 100 micro m only a few cells were obsd. [on SciFinder (R)]

Details

Item Type Articles
CreatorsBodamyali, T., Jarman-Smith, M., Stevens, C. and Chaudhuri, J.
Uncontrolled Keywordsbiol (biological study), prp (properties), fibroblast (dermal, quantifying human dermal fibroblast migration through collagen matrixes), human (quantifying human dermal fibroblast migration through collagen matrixes), thu (therapeutic use), uses (uses) (quantifying human dermal fibroblast migration through collagen matrixes), prosthetic materials and prosthetics (scaffolds, skin (fibroblast, porosity (quantifying cell migration through tissue engineering scaffolds), cell migration, collagen scaffold fibroblast migration quantification, collagens role, quantifying cell migration through tissue engineering scaffolds)
DepartmentsFaculty of Engineering & Design > Chemical Engineering
RefereedYes
StatusPublished
ID Code1017

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