Dukes, J. D., 2008. Investigating the link between phosphoinositides, endosomal trafficking and ESCRT function. Thesis (Doctor of Philosophy (PhD)). University of Bath.
The maturation of early endosomes into multivesicular bodies (MVBs) and subsequent trafficking to lysosomes is an important event for the control and silencing of endocytosed membrane receptors. The endosomal-sorting complex required for transport (ESCRT) proteins appear to play a key role in this event. Phosphatidylinositol lipids including PtdIns(3,5)P2 have been implicated in the MVB-lysosomal pathway and an ESCRT-III component CHMP3 binds to this lipid in vitro. The purpose of this thesis was to investigate the link between ESCRT proteins, PtdIns(3,5)P2 and endo-lysosomal trafficking. Firstly, a protein expressed by Salmonella, which is a phosphatase that acts on PtdIns(3,5)P2, was investigated as a potential tool for manipulating cellular PtdIns(3,5)P2 levels. Our results suggest that it is potentially a useful tool for this purpose and that expression of SopB perturbs endosome to lysosome trafficking. These findings provide further evidence for a role of PtdIns(3,5)P2 in endo-lysosomal trafficking.
|Item Type ||Thesis (Doctor of Philosophy (PhD))|
|Creators||Dukes, J. D.|
|Uncontrolled Keywords||mvb, pikfyve, cytokinesis, phosphoinositides, endosome trafficking, escrt|
|Departments||Faculty of Science > Biology & Biochemistry|
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