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Investigating the link between phosphoinositides, endosomal trafficking and ESCRT function


Reference:

Dukes, J. D., 2008. Investigating the link between phosphoinositides, endosomal trafficking and ESCRT function. Thesis (Doctor of Philosophy (PhD)). University of Bath.

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    Abstract

    The maturation of early endosomes into multivesicular bodies (MVBs) and subsequent trafficking to lysosomes is an important event for the control and silencing of endocytosed membrane receptors. The endosomal-sorting complex required for transport (ESCRT) proteins appear to play a key role in this event. Phosphatidylinositol lipids including PtdIns(3,5)P2 have been implicated in the MVB-lysosomal pathway and an ESCRT-III component CHMP3 binds to this lipid in vitro. The purpose of this thesis was to investigate the link between ESCRT proteins, PtdIns(3,5)P2 and endo-lysosomal trafficking. Firstly, a protein expressed by Salmonella, which is a phosphatase that acts on PtdIns(3,5)P2, was investigated as a potential tool for manipulating cellular PtdIns(3,5)P2 levels. Our results suggest that it is potentially a useful tool for this purpose and that expression of SopB perturbs endosome to lysosome trafficking. These findings provide further evidence for a role of PtdIns(3,5)P2 in endo-lysosomal trafficking.

    Details

    Item Type Thesis (Doctor of Philosophy (PhD))
    CreatorsDukes, J. D.
    Uncontrolled Keywordsmvb, pikfyve, cytokinesis, phosphoinositides, endosome trafficking, escrt
    DepartmentsFaculty of Science > Biology & Biochemistry
    StatusPublished
    ID Code11371

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