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The structure of Sulfolobus solfataricus 2-keto-3-deoxygluconate kinase


Reference:

Potter, J. A., Kerou, M., Lamble, H. J., Bull, S. D., Hough, D. W., Danson, M. J. and Taylor, G. L., 2008. The structure of Sulfolobus solfataricus 2-keto-3-deoxygluconate kinase. Acta Crystallographica Section D-Biological Crystallography, 64, pp. 1283-1287.

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Official URL:

http://dx.doi.org/10.1107/S0907444908036111

Abstract

The hyperthermophilic archaeon Sulfolobus solfataricus grows optimally above 353 K and utilizes an unusual promiscuous nonphosphorylative Entner-Doudoroff pathway to metabolize both glucose and galactose. It has been proposed that a part-phosphorylative Entner-Doudoroff pathway occurs in parallel in S. solfataricus, in which the 2-keto-3-deoxygluconate kinase (KDGK) is promiscuous for both glucose and galactose metabolism. Recombinant S. solfataricus KDGK protein was expressed in Escherichia coli, purified and crystallized in 0.1 M sodium acetate pH 4.1 and 1.4 M NaCl. The crystal structure of apo S. solfataricus KDGK was solved by molecular replacement to a resolution of 2.0 A and a ternary complex with 2-keto-3-deoxygluconate (KDGlu) and an ATP analogue was resolved at 2.1 angstrom. The complex suggests that the structural basis for the enzyme's ability to phosphorylate KDGlu and 2-keto-3-deoxygalactonate (KDGal) is derived from a subtle repositioning of residues that are conserved in homologous nonpromiscuous kinases.

Details

Item Type Articles
CreatorsPotter, J. A., Kerou, M., Lamble, H. J., Bull, S. D., Hough, D. W., Danson, M. J. and Taylor, G. L.
DOI10.1107/S0907444908036111
DepartmentsFaculty of Science > Chemistry
Faculty of Science > Biology & Biochemistry
Research CentresCentre for Extremophile Research (CER)
RefereedYes
StatusPublished
ID Code12335

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