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Boronate affinity saccharide electrophoresis: A novel carbohydrate analysis tool


Reference:

Jackson, T. R., Springall, J. S., Rogalle, D., Masurnoto, N., Li, H. C., D'Hooge, F., Perera, S. P., Jenkins, A. T. A., James, T. D., Fossey, J. S. and van den Elsen, J. M. H., 2008. Boronate affinity saccharide electrophoresis: A novel carbohydrate analysis tool. Electrophoresis, 29 (20), pp. 4185-4191.

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Official URL:

http://dx.doi.org/10.1002/elps.200800178

Abstract

The incorporation of specialised carbohydrate affinity ligand methacrylamido phenylboronic acid in polyacrylamide gels for fluorophore-assisted carbohydrate electrophoresis greatly improved the effective separation of saccharides that show similar mobilities in standard electrophoresis. Polyacrylamide gel electrophoresis using methacrylamido phenylboronic acid in low loading (typically 0.5-1% dry weight) was unequivocally shown to alter retention of labelled saccharides depending on their boronate affinity. While conventional fluorophore-assisted carbohydrate electrophoresis of 2-aminoacridone labelled glucose oligomers showed an inverted parabolic migration, an undesired trait of small oligosaccharides labelled with this neutral fluorophore, boron affinity saccharide electrophoresis separation of these carbohydrates completely restored their predicted running order, based on their charge/mass ratio, and resulted in improved separation of the analyte saccharides. These results exemplify boron affinity saccharide electrophoresis as an important new technique for analysing carbohydrates and sugar-containing molecules.

Details

Item Type Articles
CreatorsJackson, T. R., Springall, J. S., Rogalle, D., Masurnoto, N., Li, H. C., D'Hooge, F., Perera, S. P., Jenkins, A. T. A., James, T. D., Fossey, J. S. and van den Elsen, J. M. H.
DOI10.1002/elps.200800178
Uncontrolled Keywordscarbohydrate affinity electrophoresis, boronic acid, fluorophore-assisted carbohydrate electrophoresis
DepartmentsFaculty of Engineering & Design > Chemical Engineering
Faculty of Science > Pharmacy & Pharmacology
Faculty of Science > Chemistry
Faculty of Science > Biology & Biochemistry
RefereedYes
StatusPublished
ID Code12402

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