Research

Extraction of amino acids by reverse iontophoresis: Simulation of therapeutic monitoring in vitro


Reference:

Sieg, A., Jeanneret, F., Fathi, M., Hochstrasser, D., Rudaz, S., Veuthey, J. L., Guy, R. H. and Delgado-Charro, M. B., 2008. Extraction of amino acids by reverse iontophoresis: Simulation of therapeutic monitoring in vitro. European Journal of Pharmaceutics and Biopharmaceutics, 70 (3), pp. 908-913.

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Official URL:

http://dx.doi.org/10.1016/j.ejpb.2008.07.003

Abstract

Reverse iontophoresis across the skin has been investigated as alternative, non-invasive method for clinical and therapeutic drug monitoring. This research investigated the reverse iontophoretic extraction of 19 amino acids present at clinically relevant levels in the subdermal compartment of an in vitro diffusion cell. Over a simulated, systemic concentration range of 0-500 mu M, the extraction of amino acids was linear. Charged amino acids were extracted towards the electrode of opposite polarity, while zwitterionic species were extracted to both anode and cathode with the latter predominating. The reverse iontophoretic extraction flux was a linear function of amino acid isoelectric point, reflecting the different contributions of electromigration and electroosmosis to electrotrans port. Overall, the results confirm the feasibility of monitoring amino acids at clinically relevant levels and provide an incentive for in vivo research to further explore the clinical potential of reverse iontophoresis for the non-invasive monitoring of amino acids.

Details

Item Type Articles
CreatorsSieg, A., Jeanneret, F., Fathi, M., Hochstrasser, D., Rudaz, S., Veuthey, J. L., Guy, R. H. and Delgado-Charro, M. B.
DOI10.1016/j.ejpb.2008.07.003
Uncontrolled Keywordsskin, amino acids, clinical chemistry, reverse iontophoresis, therapeutic monitoring, non-invasive, phenylalanine
DepartmentsFaculty of Science > Pharmacy & Pharmacology
RefereedYes
StatusPublished
ID Code12442

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