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Extraction, structural characterisation and evaluation of hydroxycinnamate esters of orchard grass (Dactylis glomerata) as substrates for polyphenol oxidase


Reference:

Parveen, I., Winters, A., Threadgill, M. D., Hauck, B. and Morris, P., 2008. Extraction, structural characterisation and evaluation of hydroxycinnamate esters of orchard grass (Dactylis glomerata) as substrates for polyphenol oxidase. Phytochemistry, 69 (16), pp. 2799-2806.

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http://dx.doi.org/10.1016/j.phytochem.2008.08.019

Abstract

Polyphenol oxidase (PPO) activity has been reported in orchard grass (Dactylis glomerata); however, to date, no endogenous substrates have been identified. In the present study, we report the isolation and structural elucidation of PPO substrates in this species. The free phenol fraction was extracted, separated by reverse-phase chromatography and six potential substrates, including two hydroxycinnamate esters, were identified by UV spectrometry, electrospray ionisation-tandem mass spectrometry (LC-ESI-MSn) and 1D and 2D NMR analyses (H-1 NMR, C-13 NMR, DEPT, COSY, HMQC and HMBC). Furthermore, three caffeoylquinic acids (3-CQA, 4-CQA and 5-CQA) were identified by comparison of their spectral data (ESI-MS) with those of known compounds and literature data. Five of these compounds were demonstrated to be substrates for orchard grass PPO. (C) 2008 Elsevier Ltd. All rights reserved.

Details

Item Type Articles
CreatorsParveen, I., Winters, A., Threadgill, M. D., Hauck, B. and Morris, P.
DOI10.1016/j.phytochem.2008.08.019
Uncontrolled Keywordsesters, orchard grass, dactylis glomerata, threonic acid, isocitric acid, hydroxycinnamate, hydroxycitric acid, polyphenol oxidase
DepartmentsFaculty of Science > Pharmacy & Pharmacology
RefereedYes
StatusPublished
ID Code12443

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