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The Staphylococcus aureus Protein Sbi Acts as a Complement Inhibitor and Forms a Tripartite Complex with Host Complement Factor H and C3b


Reference:

Haupt, K., Reuter, M., van den Elsen, J. M. H., Burman, J., Halbich, S., Richter, J., Skerka, C. and Zipfel, P. F., 2008. The Staphylococcus aureus Protein Sbi Acts as a Complement Inhibitor and Forms a Tripartite Complex with Host Complement Factor H and C3b. PLoS Pathogens, 4 (12), e1000250.

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    Official URL:

    http://dx.doi.org/10.1371/journal.ppat.1000250

    Abstract

    The Gram-positive bacterium Staphylococcus aureus, similar to other pathogens, binds human complement regulators Factor H and Factor H related protein 1 (FHR-1) from human serum. Here we identify the secreted protein Sbi (Staphylococcus aureus binder of IgG) as a ligand that interacts with Factor H by a-to our knowledge-new type of interaction. Factor H binds to Sbi in combination with C3b or C3d, and forms tripartite Sbi: C3: Factor H complexes. Apparently, the type of C3 influences the stability of the complex; surface plasmon resonance studies revealed a higher stability of C3d complexed to Sbi, as compared to C3b or C3. As part of this tripartite complex, Factor H is functionally active and displays complement regulatory activity. Sbi, by recruiting Factor H and C3b, acts as a potent complement inhibitor, and inhibits alternative pathway-mediated lyses of rabbit erythrocytes by human serum and sera of other species. Thus, Sbi is a multifunctional bacterial protein, which binds host complement components Factor H and C3 as well as IgG and beta 2-glycoprotein I and interferes with innate immune recognition.

    Details

    Item Type Articles
    CreatorsHaupt, K., Reuter, M., van den Elsen, J. M. H., Burman, J., Halbich, S., Richter, J., Skerka, C. and Zipfel, P. F.
    DOI10.1371/journal.ppat.1000250
    DepartmentsFaculty of Science > Biology & Biochemistry
    Publisher Statementjournal.ppat.1000250.pdf: Citation: Haupt K, Reuter M, van den Elsen J, Burman J, Hälbich S, et al. (2008) The Staphylococcus aureus Protein Sbi Acts as a Complement Inhibitor and Forms a Tripartite Complex with Host Complement Factor H and C3b. PLoS Pathog 4(12): e1000250. doi:10.1371/journal.ppat.1000250 © 2008 Haupt et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
    RefereedYes
    StatusPublished
    ID Code13874

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