Haupt, K., Reuter, M., van den Elsen, J. M. H., Burman, J., Halbich, S., Richter, J., Skerka, C. and Zipfel, P. F., 2008. The Staphylococcus aureus Protein Sbi Acts as a Complement Inhibitor and Forms a Tripartite Complex with Host Complement Factor H and C3b. PLoS Pathogens, 4 (12), e1000250.
The Gram-positive bacterium Staphylococcus aureus, similar to other pathogens, binds human complement regulators Factor H and Factor H related protein 1 (FHR-1) from human serum. Here we identify the secreted protein Sbi (Staphylococcus aureus binder of IgG) as a ligand that interacts with Factor H by a-to our knowledge-new type of interaction. Factor H binds to Sbi in combination with C3b or C3d, and forms tripartite Sbi: C3: Factor H complexes. Apparently, the type of C3 influences the stability of the complex; surface plasmon resonance studies revealed a higher stability of C3d complexed to Sbi, as compared to C3b or C3. As part of this tripartite complex, Factor H is functionally active and displays complement regulatory activity. Sbi, by recruiting Factor H and C3b, acts as a potent complement inhibitor, and inhibits alternative pathway-mediated lyses of rabbit erythrocytes by human serum and sera of other species. Thus, Sbi is a multifunctional bacterial protein, which binds host complement components Factor H and C3 as well as IgG and beta 2-glycoprotein I and interferes with innate immune recognition.
|Item Type ||Articles|
|Creators||Haupt, K., Reuter, M., van den Elsen, J. M. H., Burman, J., Halbich, S., Richter, J., Skerka, C. and Zipfel, P. F.|
|Departments||Faculty of Science > Biology & Biochemistry|
|Publisher Statement||journal.ppat.1000250.pdf: Citation: Haupt K, Reuter M, van den Elsen J, Burman J, Hälbich S, et al. (2008) The Staphylococcus aureus Protein Sbi Acts as a Complement Inhibitor and Forms a Tripartite Complex with Host Complement Factor H and C3b. PLoS Pathog 4(12): e1000250. doi:10.1371/journal.ppat.1000250 © 2008 Haupt et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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