Formation of a human-derived fat tissue layer in PDLLGA hollow fibre scaffolds for adipocyte tissue engineering

Reference:

Morgan, S. M., Ainsworth, B. J., Kanczler, J. M., Babister, J. C., Chaudhuri, J. B. and Oreffo, R. O. C., 2009. Formation of a human-derived fat tissue layer in PDLLGA hollow fibre scaffolds for adipocyte tissue engineering. Biomaterials, 30 (10), pp. 1910-1917.

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Official URL:

http://dx.doi.org/10.1016/j.biomaterials.2008.12.033

Abstract

Development of adipose tissue-engineering strategies, where human bone marrow stromal cells (HBMSC) are combined with three-dimensional scaffolds, is likely to prove valuable for soft tissue restoration. In this study, we assessed the function of poly(DL-lactide-co-glycolide) (PDLLGA) hollow fibres in facilitating the development of HBMSC-derived adipocytes for advancement of an associated adipocyte layer. The large surface area of 75:25 PDLLGA fibres facilitated the rapid generation of extensive adipocyte aggregates from an undifferentiated HBMSC monolayer, where the fat-laden cells stained positive with Oil Red O and expressed the adipocyte marker, fatty acid binding protein 3 (FABP3). Following implantation subcutaneously in severely compromised immunodeficient mice, the adipogenic phenotype of the PLGA-adipocyte graft was maintained for up to 56 days. Confocal microscopy showed associated LipidTOX (TM) Deep Red neutral lipid staining in an FLPDLGA fibre-adipocyte graft after 56 days, critical evidence demonstrating maintenance of the adipocyte phenotype in the subcutaneous graft. To support adipose tissue advancement in a defined volume, the PDLLGA-adipocyte scaffold was encapsulated within alginate/chitosan hydrogel capsules (typical diameters, 4.0 mm). In a 28-day in vivo trial in immunodeficient mice, clusters of the capsules were maintained at the subcutaneous site. An adipocyte tissue layer advancing within the surrounding hydrogel was demonstrated.

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