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Structures of native human thymidine phosphorylase and in complex with 5-iodouracil


Reference:

Mitsiki, E., Papageorgiou, A. C., Iyer, S., Thiyagarajan, N., Prior, S. H., Sleep, D., Finnis, C. and Acharya, K. R., 2009. Structures of native human thymidine phosphorylase and in complex with 5-iodouracil. Biochemical and Biophysical Research Communications, 386 (4), pp. 666-670.

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Official URL:

http://dx.doi.org/10.1016/j.bbrc.2009.06.104

Abstract

Thymidine phosphorylase (TP) first identified as platelet derived endothelial cell growth factor (PD-ECGF) plays a key role in nucleoside metabolism. Human TP (hTP) is implicated in angiogenesis and is overexpressed in several solid tumors. Here, we report the crystal structures of recombinant hTP and its complex with a substrate 5-iodouracil (5IUR) at 3.0 and 2.5 angstrom, respectively. in addition, we provide information on the role of specific residues in the enzymatic activity of hTP through mutagenesis and kinetic Studies.

Details

Item Type Articles
CreatorsMitsiki, E., Papageorgiou, A. C., Iyer, S., Thiyagarajan, N., Prior, S. H., Sleep, D., Finnis, C. and Acharya, K. R.
DOI10.1016/j.bbrc.2009.06.104
DepartmentsFaculty of Science > Biology & Biochemistry
RefereedYes
StatusPublished
ID Code15291

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