DNase1 footprints suggest the involvement of at least three types of transcription factors in the regulation of α-Amy2/A by gibberellin
Willmott, R. L., Rushton, P. J., Hooley, R. and Lazarus, C. M., 1998. DNase1 footprints suggest the involvement of at least three types of transcription factors in the regulation of α-Amy2/A by gibberellin. Plant Molecular Biology, 38 (5), pp. 817-825.
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To elucidate the mechanisms by which α-amylase genes are expressed in wild oat aleurone, two genes, α-Amy2/A and α-Amy2/D, were isolated. Both were shown to be positively regulated by gibberellin (GA) during germination and both contain the conserved cis-acting elements Box 2, GA-response element (TAACAGA) and TATCSATSS (where S is C or G). In addition, they possess a conserved initiator element (CATCA) that is present in both α-Amy2 and α-Amy1 genes, and also in a number of other plans TATA-containing and TATA-less promoters. DNase1 footprint analysis showed the α-Amy2/A promoter to be a complex array of binding sites for a number of different classes of DNA-binding proteins. Our data suggest that the area around the initiator element (Inr) is bound by a large complex of general transcription factors, that the TATA box is bound by the TFIID complex, that Box 2 is bound by one or more WRKY proteins and that the GA-response element is bound by one or more MYBs. TWO other elements containing the core sequence CCATGG/C are bound by nuclear protein and this sequence is the core of the Sph element. The regulation of α-Amy2 genes by GA therefore involves an interplay of at least three different types of transcription factor.
|Creators||Willmott, R. L., Rushton, P. J., Hooley, R. and Lazarus, C. M.|
|Uncontrolled Keywords||abscisic-acid, promoter, binding proteins, l aleurone protoplasts, plasma-membrane, tata box, initiator element, aleurone, response complex, dna-binding proteins, barley, cis-acting elements, alpha-amylase gene, plant genes|
|Departments||Faculty of Science > Biology & Biochemistry|
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