Research

Re-partitioning of Cu and Zn isotopes by modified protein expression


Reference:

Büchl, A., Hawkesworth, C., Ragnarsdottir, K. V. and Brown, D. R., 2008. Re-partitioning of Cu and Zn isotopes by modified protein expression. Geochemical Transactions, 9 (11).

Related documents:

[img]
Preview
PDF (1467-4866-9-11.pdf) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (335kB) | Preview

    Official URL:

    http://dx.doi.org/10.1186/1467-4866-9-11

    Abstract

    Cu and Zn have naturally occurring non radioactive isotopes, and their isotopic systematics in a biological context are poorly understood. In this study we used double focussing mass spectroscopy to determine the ratios for these isotopes for the first time in mouse brain. The Cu and Zn isotope ratios for four strains of wild-type mice showed no significant difference (δ65Cu - 0.12 to -0.78 permil; δ66Zn -0.23 to -0.48 permil). We also looked at how altering the expression of a single copper binding protein, the prion protein (PrP), alters the isotope ratios. Both knockout and overexpression of PrP had no significant effect on the ratio of Cu isotopes. Mice brains expressing mutant PrP lacking the known metal binding domain have δ65Cu isotope values of on average 0.57 permil higher than wild-type mouse brains. This implies that loss of the copper binding domain of PrP increases the level of 65Cu in the brain. δ66Zn isotope values of the transgenic mouse brains are enriched for 66Zn to the wild-type mouse brains. Here we show for the first time that the expression of a single protein can alter the partitioning of metal isotopes in mouse brains. The results imply that the expression of the prion protein can alter cellular Cu isotope content.

    Details

    Item Type Articles
    CreatorsBüchl, A., Hawkesworth, C., Ragnarsdottir, K. V. and Brown, D. R.
    DOI10.1186/1467-4866-9-11
    DepartmentsFaculty of Science > Biology & Biochemistry
    Publisher Statement1467-4866-9-11.pdf: © 2008 Büchl et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
    RefereedYes
    StatusPublished
    ID Code18016

    Export

    Actions (login required)

    View Item

    Document Downloads

    More statistics for this item...