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Diol appended quenchers for fluorescein boronic acid


Reference:

Elfeky, S. A., Flower, S. E., Masumoto, N., D'Hooge, F., Labarthe, L., Chen, W. B., Len, C., James, T. D. and Fossey, J. S., 2010. Diol appended quenchers for fluorescein boronic acid. Chemistry - An Asian Journal, 5 (3), pp. 581-588.

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Official URL:

http://dx.doi.org/10.1002/asia.200900386

Abstract

Fluorescein isothiocyanate is treated with 3-aminophenylboronic acid to provide a fluorescently tagged boronic acid derivative which is used to assess Forster resonance energy transfer (FRET) quenching upon boronate ester formation with a series of bespoke diol appended quenchers. Fluorescence spectroscopy comparison of quenching efficiency between treatment of fluorescein and its boronic acid appended congener with quencher appended diol reveals boronate ester formation (covalently linked) to be the more efficient regime and from the panel of quenchers which also included nucleosides.

Details

Item Type Articles
CreatorsElfeky, S. A., Flower, S. E., Masumoto, N., D'Hooge, F., Labarthe, L., Chen, W. B., Len, C., James, T. D. and Fossey, J. S.
DOI10.1002/asia.200900386
DepartmentsFaculty of Science > Chemistry
RefereedYes
StatusPublished
ID Code18454

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