Research

Label-free sub-picomolar protein detection with field-effect transistors


Reference:

Estrela, P., Paul, D., Song, Q., Stadler, L., Wang, L., Huq, E., Davis, J., Ko Ferrigno, P. and Migliorato, P., 2010. Label-free sub-picomolar protein detection with field-effect transistors. Analytical Chemistry, 82 (9), pp. 3531-3536.

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    Official URL:

    http://dx.doi.org/10.1021/ac902554v

    Abstract

    Proteins mediate the bulk of biological activity and are powerfully assayed in the diagnosis of diseases. Protein detection relies largely on antibodies, which have significant technical limitations especially when immobilized on two-dimensional surfaces. Here, we report the integration of peptide aptamers with extended gate metal-oxide-semiconductor field-effect transistors (MOSFETs) to achieve label-free sub-picomolar target protein detection. Specifically, peptide aptamers that recognize highly related protein partners of the cyclin-dependent kinase (CDK) family are immobilized on the transistor gate to enable human CDK2 to be detected at 100 fM or 5 pg/mL, well within the clinically relevant range. The target specificity, ease of fabrication, and scalability of these FET arrays further demonstrate the potential application of the multiplexable field effect format to protein sensing.

    Details

    Item Type Articles
    CreatorsEstrela, P., Paul, D., Song, Q., Stadler, L., Wang, L., Huq, E., Davis, J., Ko Ferrigno, P. and Migliorato, P.
    DOI10.1021/ac902554v
    DepartmentsFaculty of Engineering & Design > Electronic & Electrical Engineering
    Research CentresCentre for Advanced Sensor Technologies (CAST)
    Publisher StatementEstrela_Analytical-Chemistry_2010_92_9_3531.pdf: This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/ac902554v
    RefereedYes
    StatusPublished
    ID Code18721

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