Autoantibodies to the collagenous region of C1q occur in three strains of lupus-prone mice
Hogarth, M., Norsworthy, P., Allen, P., Trinder, P., Loos, M., Morley, B. J., Walport, M. and Davies, K., 1996. Autoantibodies to the collagenous region of C1q occur in three strains of lupus-prone mice. Clinical And Experimental Immunology, 104 (2), pp. 241-246.
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We have developed an ELISA to measure murine autoantibodies to the collagenous region (CLR) of Clq, using the whole human Clq molecule as the solid-phase ligand, in the presence of 1 M NaCl. The assay was validated by testing positive sera from 20 mice using purified mouse Clq, and from 10 mice using purified human Clq-CLR, as the solid-phase ligands. There were highly significant correlations between results obtained with human Clq(whole molecule) and: (i) mouse Clq (r(Sp) = 0.73, P < 0.001), and (ii) human Clq-CLR alone (r(Sp) = 0.86, P = 0.001). Antibodies to Clq were measured in 53 MRL/lpr, 17 BXSB and 25 NZB/W lupus-prone mice. Median (range) anti-Clq (CLR) antibody levels in MRL/lpr, BXSB, and NZB/W autoimmune mice aged 3 months were 22 (16-66), 21 (17-39) and 19 (15-27) EU, respectively. The median anti-Clq antibody level in MRL/lpr mice aged 5 months was 76 (35-142) EU, significantly higher than that at 3 months (U = 558, P < 0.0005). Median anti-Clq antibody level in NZB/W mice at 8 months was 37 (13-74) EU and in BXSB mice at 11 months was 62 (31-231) EU, significantly higher than corresponding values at 3 months (U = 326, and U = 4, P < 0.001, respectively). This is the first demonstration of anti-Clq (CLR) antibodies in NZB/W and BXSB mice. The pathologic significance and the potential utility of these antibodies for monitoring disease in lupus-prone mice are under evaluation.
|Creators||Hogarth, M., Norsworthy, P., Allen, P., Trinder, P., Loos, M., Morley, B. J., Walport, M. and Davies, K.|
|Departments||University Administration & Central Services > Vice-Chancellor's Office|
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