Effects of polyelectrolyte complex micelles and their components on the enzymatic activity of lipase
Lindhoud, S., Norde, W. and Stuart, M. A. C., 2010. Effects of polyelectrolyte complex micelles and their components on the enzymatic activity of lipase. Langmuir, 26 (12), pp. 9802-9808.
Related documents:This repository does not currently have the full-text of this item.
You may be able to access a copy if URLs are provided below. (Contact Author)
The enzymatic activity of Hi-lipase embedded in complexes of poly-2-methylvinylpyridinium-co-poly(ethylene oxide) (P2MVP(41)-PEG(205)) and poly(acrylic acid)(PAA(139)) is studied as a function of the PAA(139) + P2MVP(41) - PEO205 complex composition. The measurements revealed that there are several factors that influence the enzymatic activity. When incorporated in micelles, the activity of lipase is increased, which suggests that the micelles favor the active state. The activity may further increase because the substrate tends to accumulate to the micelles. It is found that the presence of PAA(139) alone also increases the enzymatic activity somewhat. Increasing of the ionic strength decreases the enzymatic activity in all systems. However, at ionic strengths where the micelles are disintegrated (>0.5 M), the activity of lipase in the presence of both polyelectrolytes is still higher than the activity of free lipase. At 0.7 M NaCl it was found that lipase in the presence of (just) P2MVP(41) - PEO205 is more active than lipase without this additive.
|Creators||Lindhoud, S., Norde, W. and Stuart, M. A. C.|
|Departments||Faculty of Science > Chemistry|
Actions (login required)