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Microemulsion formulations for the transdermal delivery of testosterone


Reference:

Hathout, R. M., Woodman, T. J., Mansour, S., Mortada, N. D., Geneidi, A. S. and Guy, R. H., 2010. Microemulsion formulations for the transdermal delivery of testosterone. European Journal of Pharmaceutical Sciences, 40 (3), pp. 188-196.

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    Official URL:

    http://dx.doi.org/10.1016/j.ejps.2010.03.008

    Abstract

    The objective was to develop a microemulsion formulation for the transdermal delivery of testosterone. Microemulsion formulations were prepared using oleic acid as the oil phase, Tween20 as a surfactant, Transcutol (R) as cosurfactant, and water. The microemulsions were characterized visually, with the polarizing microscope, and by dynamic light scattering. In addition, the pH, conductivity (sigma) and viscosity (eta) of the formulations were measured. Moreover, differential scanning calorimetry and diffusion-ordered nuclear magnetic resonance spectroscopy were used to study the formulations investigated. Conductivity measurements revealed, as a function of the weight fraction of the aqueous phase, the point at which the microemulsion made the transition from water-in-oil to bicontinuous. Alterations in the microstructure of the microemulsions, following incorporation of testosterone, have been evaluated using the same physical parameters (pH, sigma and eta) and via Fourier-transform infrared spectroscopy (FT-IR), H-1 NMR and C-13 NMR. These methods were also used to determine the location of the drug in the colloidal formulation. Finally, testosterone delivery from selected formulations was assessed across porcine skin in vitro in Franz diffusion cells. The physical parameter determinations, combined with the spectroscopic studies, demonstrated that the drug was principally located in the oily domains of the microemulsions. Testosterone was delivered successfully across the skin from the microemulsions examined, with the highest flux achieved (4.6 +/- 0.6 mu g cm(-2) h(-1)) from a formulation containing 3% (w/v) of the active drug and the composition (w/w) of 16% oleic acid, 32% Tween20, 32% Transcutol (R) and 20% water. The microemulsions considered offer potentially useful vehicles for the transdermal delivery of testosterone.

    Details

    Item Type Articles
    CreatorsHathout, R. M., Woodman, T. J., Mansour, S., Mortada, N. D., Geneidi, A. S. and Guy, R. H.
    DOI10.1016/j.ejps.2010.03.008
    Uncontrolled Keywordstransdermal drug delivery, h-1 nmr, testosterone, microemulsions, skin permeation
    DepartmentsFaculty of Science > Pharmacy & Pharmacology
    Publisher StatementEur_J_Pharm_Sci_40_188-196_2010.pdf: A definitive version of this article is published in European Journal of Pharmaceutical Sciences, [VOL 40, ISSUE 3], 14 June 2010, http://dx.doi.org/10.1016/j.ejps.2010.03.008.
    RefereedYes
    StatusPublished
    ID Code19539

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