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Quantitative determination of 5-aminolaevulinic acid and its esters in cell lysates by HPLC-fluorescence


Reference:

Giuntini, F., Bourre, L., MacRobert, A. J., Wilson, M. and Eggleston, I. M., 2008. Quantitative determination of 5-aminolaevulinic acid and its esters in cell lysates by HPLC-fluorescence. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 875 (2), pp. 562-566.

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Official URL:

http://dx.doi.org/10.1016/j.jchromb.2008.09.031

Abstract

The development of a reliable sensitive method for the HPLC determination of 5-aminolaevulinic acid (ALA) and ALA esters in cell lysates is described. The method relies on the quantification of a fluorescent derivative of ALA following its derivatisation with acetylacetone and formaldehyde. Following this procedure it is possible to quantify ALA in cell lysates with no need for pre-purification of the sample. The method has been validated in two ranges of concentration (0.6-65 mu M, 0.1-10 mu g/mL, and 30-600 mu M, 5-100 mu g/mL), and has also been extended and validated for the determination of ALA released from ALA prodrugs after acidic hydrolysis.

Details

Item Type Articles
CreatorsGiuntini, F., Bourre, L., MacRobert, A. J., Wilson, M. and Eggleston, I. M.
DOI10.1016/j.jchromb.2008.09.031
DepartmentsFaculty of Science > Pharmacy & Pharmacology
RefereedYes
StatusPublished
ID Code19806

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