Research

Evidence for PI3K-dependent CXCR3 agonist-induced degranulation of human cord blood-derived mast cells


Reference:

Willox, I., Mirkina, I., Westwick, J. and Ward, S. G., 2010. Evidence for PI3K-dependent CXCR3 agonist-induced degranulation of human cord blood-derived mast cells. Molecular Immunology, 47 (14), pp. 2367-2377.

Related documents:

This repository does not currently have the full-text of this item.
You may be able to access a copy if URLs are provided below. (Contact Author)

Official URL:

http://dx.doi.org/10.1016/j.molimm.2010.05.005

Abstract

The chemokine receptor CXCR3, which has three known variants (CXCR3-A, CXCR3-B and CXCR3-Alt), has been implicated in the recruitment of mast cells to tissues in many different chronic diseases with its agonists found in elevated levels in several pulmonary diseases. All three variants of CXCR3 were detected in cord blood-derived mast cells at the mRNA level. Using an antibody that is unable to distinguish individual CXCR3 isoforms, we detected a marked down-regulation of intracellular protein during maturation from progenitor cells, with no concomitant changes in the modest surface expression of CXCR3. The known CXCR3 agonists CXCL9, CXCL10 and CXCL11 as well as the reported CXCR3-B agonist CXCL4, were able to induce Akt and ERK1/2 phosphorylation, as well as partial degranulation. Responses to all agonists were inhibited by pre-treatment with selective CXCR3 antagonists and pertussis toxin. Use of novel isoform-selective inhibitors, indicates that the p110γ isoform of PI3K is required for degranulation and signaling responses to CXCR3 agonists.

Details

Item Type Articles
CreatorsWillox, I., Mirkina, I., Westwick, J. and Ward, S. G.
DOI10.1016/j.molimm.2010.05.005
DepartmentsFaculty of Science > Pharmacy & Pharmacology
RefereedYes
StatusPublished
ID Code20041

Export

Actions (login required)

View Item