Detection of molecular interactions with modified ferrocene self-assembled monolayers

Reference:

Ho, M. Y., Li, P., Estrela, P., Goodchild, S. and Migliorato, P., 2010. Detection of molecular interactions with modified ferrocene self-assembled monolayers. Journal of Physical Chemistry B, 114 (32), pp. 10661-10665.

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Official URL:

http://dx.doi.org/10.1021/jp104560e

Abstract

Ferrocene-terminated self-assembled monolayers (Fc-SAMs) are one of the most studied molecular aggregates on metal electrodes. They are easy to fabricate and provide a stable and reproducible system to investigate the effect of the microenvironment on the electron transfer parameters. We propose a novel application for Fc-SAMs, the detection of molecular interactions, based on the modification of the SAM with target-specific receptors. Mixed SAMs were fabricated by coimmobilization on Au electrodes of thiolated alkane chains with three different head groups: hydroxy terminating head group, ferrocene head group, and a functional head group such as biotin. Upon binding, the intrinsic electric charge of the target (e.g., streptavidin) modifies the electrostatic potential at the plane of electron transfer, causing a shift in the formal potential E°′. The SAMs were characterized by AC voltammetry. The detection mechanism is confirmed by measurements of formal potential as a function of electrolyte pH.

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