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Isolation and culture of adult mouse hepatocytes


Reference:

Li, W.-C., Ralphs, K. L. and Tosh, D., 2010. Isolation and culture of adult mouse hepatocytes. In: Ward, A. and Tosh, D., eds. Mouse Cell Culture: Methods and Protocols.633 ed. Humana Press, pp. 185-196. (Methods in Molecular Biology)

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Official URL:

http://dx.doi.org/10.1007/978-1-59745-019-5_13

Abstract

The liver performs a multitude of functions including the regulation of carbohydrate, fat, and protein metabolism, the detoxification of endo- and xenobiotics, and the synthesis and secretion of plasma proteins and bile. Isolated hepatocytes constitute a useful technique for studying liver function in an in vitro setting. Here we describe a method for the isolation of hepatocytes from adult mouse liver. The principle of the method is the two-step collagenase perfusion technique which involves sequential perfusion of the liver with ethylenediaminetetraacetic acid and collagenase. Following isolation, the cells can either be used for short-term studies or, alternatively, maintained in culture for prolonged periods to study long-term changes in gene expression. The protocol for mouse hepatocyte isolation may be applied to both normal and transgenic mice.

Details

Item Type Book Sections
CreatorsLi, W.-C., Ralphs, K. L. and Tosh, D.
EditorsWard, A.and Tosh, D.
DOI10.1007/978-1-59745-019-5_13
DepartmentsFaculty of Science > Biology & Biochemistry
RefereedNo
StatusPublished
ID Code20342

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