Isolation and culture of adult mouse hepatocytes
Li, W.-C., Ralphs, K. L. and Tosh, D., 2010. Isolation and culture of adult mouse hepatocytes. In: Ward, A. and Tosh, D., eds. Mouse Cell Culture: Methods and Protocols.633 ed. Humana Press, pp. 185-196. (Methods in Molecular Biology)
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The liver performs a multitude of functions including the regulation of carbohydrate, fat, and protein metabolism, the detoxification of endo- and xenobiotics, and the synthesis and secretion of plasma proteins and bile. Isolated hepatocytes constitute a useful technique for studying liver function in an in vitro setting. Here we describe a method for the isolation of hepatocytes from adult mouse liver. The principle of the method is the two-step collagenase perfusion technique which involves sequential perfusion of the liver with ethylenediaminetetraacetic acid and collagenase. Following isolation, the cells can either be used for short-term studies or, alternatively, maintained in culture for prolonged periods to study long-term changes in gene expression. The protocol for mouse hepatocyte isolation may be applied to both normal and transgenic mice.
|Item Type||Book Sections|
|Creators||Li, W.-C., Ralphs, K. L. and Tosh, D.|
|Editors||Ward, A.and Tosh, D.|
|Departments||Faculty of Science > Biology & Biochemistry|
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