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Isolation, culture, and characterisation of mouse embryonic oesophagus and intestine


Reference:

Quinian, J. M., Yu, W.-Y. and Tosh, D., 2010. Isolation, culture, and characterisation of mouse embryonic oesophagus and intestine. In: Ward, A. and Tosh, D., eds. Mouse Cell Culture: Methods and Protocols.633 ed. Humana Press, pp. 81-90. (Methods in Molecular Biology)

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Official URL:

http://dx.doi.org/10.1007/978-1-59745-019-5_6

Abstract

The gastrointestinal tract of vertebrates is lined by epithelium that develops from the endodermal germ layer. The oesophagus and intestine form part of the gastrointestinal tract and studying the normal development of both tissues is difficult due to lack of suitable in vitro model systems. One of the criteria for a reliable culture model includes the ability to carry out real-time observations in vitro. The method we describe here is based on the isolation of embryonic oesophagus and intestine from 11.5-day-old embryos and culture on fibronectin-coated coverslips in basal Eagle's medium and 20% fetal bovine serum. This model permits real-time observations of both tissues and over a few days in culture, markers of differentiation appear. This culture system appears to recapitulate normal oesophagus and intestine development.

Details

Item Type Book Sections
CreatorsQuinian, J. M., Yu, W.-Y. and Tosh, D.
EditorsWard, A.and Tosh, D.
DOI10.1007/978-1-59745-019-5_6
DepartmentsFaculty of Science > Biology & Biochemistry
StatusPublished
ID Code20343

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