Isolation and culture of embryonic pancreas and liver

Reference:

Burke, Z. D., Li, W.-C., Slack, J. M. and Tosh, D., 2010. Isolation and culture of embryonic pancreas and liver. In: Ward, A. and Tosh, D., eds. Mouse Cell Culture: Methods and Protocols.633 ed. Humana Press, pp. 91-99. (Methods in Molecular Biology)

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Official URL:

http://dx.doi.org/10.1007/978-1-59745-019-5_7

Abstract

Culturing embryonic tissue in an in vitro setting offers the unique ability to manipulate the external medium and therefore to investigate the pathways involved in regulating normal organogenesis as well as providing models for developmental disorders. Here we describe a system for the in vitro culture of the dorsal pancreatic buds and liver buds from mouse embryos. The tissues are dissected from day 9.0 or 11.5 mouse embryos. The tissues are placed on fibronectin-coated coverslips in serum-containing medium and allowed to attach. Over the next few days, the buds grow as flattened structures which are thin enough to allow the use of wholemount immunostaining methods.

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