Generation of medaka gene knockout models by target-selected mutagenesis

Reference:

Taniguchi, Y., Takeda, S., Furutani-Seiki, M., Kamei, Y., Todo, T., Sasado, T., Deguchi, T., Kondoh, H., Mudde, J., Yamazoe, M., Hidaka, M., Mitani, H., Toyoda, A., Sakaki, Y., Plasterk, R. H. and Cuppen, E., 2006. Generation of medaka gene knockout models by target-selected mutagenesis. Genome Biology, 7 (12), R116.

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Official URL:

http://dx.doi.org/10.1186/gb-2006-7-12-r116

Abstract

We have established a reverse genetics approach for the routine generation of medaka ( Oryzias latipes) gene knockouts. A cryopreserved library of N-ethyl-N-nitrosourea ( ENU) mutagenized fish was screened by high-throughput resequencing for induced point mutations. Nonsense and splice site mutations were retrieved for the Blm, Sirt1, Parkin and p53 genes and functional characterization of p53 mutants indicated a complete knockout of p53 function. The current cryopreserved resource is expected to contain knockouts for most medaka genes.

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