Agrobacterium-mediated transformation of Arabis gunnisoniana

Reference:

Taskin, K. M., Turgut, K., Ercan, A. G. and Scott, R. J., 2003. Agrobacterium-mediated transformation of Arabis gunnisoniana. Plant Cell Tissue and Organ Culture, 72 (2), pp. 173-179.

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Abstract

An efficient method for adventitious shoot regeneration for Arabis drummondii and a transformation protocol for A. gunnisoniana from hypocotyl explants are described. Hypocotyl explants from 7-day-old aseptically grown seedlings were cultured on MS medium containing plant growth regulators (6-benzylaminopurine, 1-phelyl-3-(1,2,3-thiadiazol-5-yl) urea, alpha-naphthaleneacetic acid and 2,4-dichlorophenoxy- acetic acid). After 4 weeks in culture, high frequency of adventitious shoot regeneration was observed. Regenerated shoots were rooted on half-strength MS basal medium supplemented 1% (w/v) sucrose, with or without NAA. This protocol was then used to produce transformed Arabis gunnisoniana plants. A. gunnisoniana hypocotyl explants were co-cultivated with Agrobacterium tumefaciens strain GV3101 harbouring pBJ40. Transgenic shoots were selected on MS medium supplemented with 50 mg l(-1) kanamycin. PCR analysis verified the presence of the nptII gene in the plant DNA isolated from kanamycin resistant shoots.

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