Research

Micro-magnetic resonance imaging study of live quail embryos during embryonic development


Reference:

Duce, S., Morrison, F., Welten, M., Baggott, G. and Tickle, C., 2011. Micro-magnetic resonance imaging study of live quail embryos during embryonic development. Magnetic Resonance Imaging, 29 (1), pp. 132-139.

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Official URL:

http://dx.doi.org/10.1016/j.mri.2010.08.004

Abstract

Eggs containing live Japanese quail embryos were imaged using micro-magnetic resonance imaging (mu MRI) at 24-h intervals from Day 0 to 8, the period during which the main body axis is being laid down and organogenesis is taking place. Considerable detail of non-embryonic structures such as the latebra was revealed at early stages but the embryo could only be visualized around Day 3. Three-dimensional (3D) changes in embryo length and volume were quantified and also changes in volume in the extra- and non-embryonic components. The embryo increased in length by 43% and nearly trebled in volume between Day 4 and Day 5. Although the amount of yolk remained fairly constant over the first 5 days, the amount of albumen decreases significantly and was replaced by extra-embryonic fluid (EEF). H-1 longitudinal (T-1) and transverse (T-2) relaxation times of different regions within the eggs were determined over the first 6 days of development. The T-2 measurements mirrored the changes in image intensity observed, which can be related to the aqueous protein concentrations. In addition, a comparison of the development of Day 0 to 3 quail embryos exposed to radiofrequency (rf) pulses, 7 T static magnetic fields and magnetic field gradients for an average of 7 h with the development of control embryos did not reveal any gross changes, thus confirming that mu MRI is a suitable tool for following the development of live avian embryos over time from the earliest stages.

Details

Item Type Articles
CreatorsDuce, S., Morrison, F., Welten, M., Baggott, G. and Tickle, C.
DOI10.1016/j.mri.2010.08.004
Uncontrolled Keywordsembryonic development, in ovo, safety, micro-mri (mu mri), embryo, quail, egg
DepartmentsFaculty of Science > Biology & Biochemistry
RefereedYes
StatusPublished
ID Code22593

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