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Using automated imaging to interrogate gonadotrophin-releasing hormone receptor trafficking and function


Reference:

Armstrong, S. P., Caunt, C. J., Finch, A. R. and McArdle, C. A., 2011. Using automated imaging to interrogate gonadotrophin-releasing hormone receptor trafficking and function. Molecular and Cellular Endocrinology, 331 (2), pp. 194-204.

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    Official URL:

    http://dx.doi.org/10.1016/j.mce.2010.07.008

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    Abstract

    Gonadotrophin-releasing hormone (GnRH) acts via seven transmembrane receptors on gonadotrophs to stimulate gonadotrophin synthesis and secretion, and thereby mediates central control of reproduction. Type I mammalian GnRHR are unique, in that they lack C-terminal tails. This is thought to underlie their resistance to rapid homologous desensitisation as well as their slow rate of internalisation and inability to provoke G-protein-independent (arrestin-mediated) signalling. More recently it has been discovered that the vast majority of human GnRHR are actually intracellular, in spite of the fact that they are activated at the cell surface by a membrane impermeant peptide hormone. This apparently reflects inefficient exit from the endoplasmic reticulum and again, the absence of the C-tail likely contributes to their intracellular localisation. This review is intended to cover some of these novel aspects of GnRHR biology, focusing on ways that we have used automated fluorescence microscopy (high content imaging) to explore GnRHR localisation and trafficking as well as spatial and temporal aspects of GnRH signalling via the Ca2+/calmodulin/calcineurin/NFAT and Raf/MEK/ERK pathways.

    Details

    Item Type Articles
    CreatorsArmstrong, S. P., Caunt, C. J., Finch, A. R. and McArdle, C. A.
    DOI10.1016/j.mce.2010.07.008
    Related URLs
    URLURL Type
    http://www.scopus.com/inward/record.url?scp=78650293760&partnerID=8YFLogxKUNSPECIFIED
    Uncontrolled Keywordspulsatility,trafficking,gonadotrophin-releasing hormone,imaging,desensitisation
    DepartmentsFaculty of Science > Biology & Biochemistry
    RefereedYes
    StatusPublished
    ID Code22993

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