A novel role for HNO in local and spreading vasodilatation in rat mesenteric resistance arteries
Yuill, K. H., Yarova, P., Kemp-Harper, B. K., Garland, C. J. and Dora, K. A., 2011. A novel role for HNO in local and spreading vasodilatation in rat mesenteric resistance arteries. Antioxidants & Redox Signaling, 14 (9), pp. 1625-1635.
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Nitric oxide-mediated vasodilatation has previously been attributed to the uncharged form of the molecule (NO center dot), but increasing evidence suggests that nitroxyl (HNO) may play a significant role in endothelium-dependent relaxation. The aim of this study was to investigate the mechanisms underlying HNO-mediated vasodilatation in phenylephrine pre-constricted pressurized (70mmHg) mesenteric arteries, and on membrane currents in isolated smooth muscle cells using whole cell and perforated patch clamp recordings. Angeli's salt (AS: nitroxyl donor), evoked concentration-dependent vasodilatation that was insensitive to the NO center dot scavengers carboxy-PTIO and hydroxocobalamin (HXC), but sensitive to either the HNO scavenger L-cysteine, K-channel blockers (4-AP and iberiotoxin), raised [K+](o), or inhibition of soluble guanylyl cyclase (ODQ). AS-evoked smooth muscle hyperpolarization significantly augmented K-V current in an ODQ sensitive manner, and also increased the BKCa current. Importantly, 30 mu M AS initiated conducted or spreading vasodilatation, and following blockade of endothelial K-channels (TRAM-34 and apamin), ACh was able to evoke similar L-cysteine-sensitive conducted dilatation. These data show that vasodilatation induced by HNO is mediated by both K-V and BKCa channels, and suggest a physiological role in vasodilatation through the vasculature.
|Creators||Yuill, K. H., Yarova, P., Kemp-Harper, B. K., Garland, C. J. and Dora, K. A.|
|Departments||Faculty of Science > Pharmacy & Pharmacology|
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