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Cloning, purification, crystallization and preliminary crystallographic analysis of the human histone deacetylase sirtuin 1


Reference:

Holbourn, K. P., Lloyd, M. D., Thompson, A. S., Threadgill, M. D. and Acharya, K. R., 2011. Cloning, purification, crystallization and preliminary crystallographic analysis of the human histone deacetylase sirtuin 1. Acta Crystallographica Section F-Structural Biology and Crystallization Communications, 67 (4), pp. 461-463.

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Official URL:

http://dx.doi.org/10.1107/s1744309111003277

Abstract

Human sirtuin 1 is a member of the histone deacetylase family and is involved in cellular aging, tumourigenesis and cellular metabolism. Recombinant sirtuin 1 comprising residues 140-747 was crystallized using the hanging-drop vapour-diffusion method. The crystal diffracted to 3.4 A resolution and belonged to space group P622, with unit-cell parameters a = b = 203.1, c = 625.3 A, and is estimated to contain between six and 12 molecules per asymmetric unit.

Details

Item Type Articles
CreatorsHolbourn, K. P., Lloyd, M. D., Thompson, A. S., Threadgill, M. D. and Acharya, K. R.
DOI10.1107/s1744309111003277
Uncontrolled Keywordssirtuin 1, histone deacetylases
DepartmentsFaculty of Science > Biology & Biochemistry
Faculty of Science > Pharmacy & Pharmacology
RefereedYes
StatusPublished
ID Code23825

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