Cloning, purification, crystallization and preliminary crystallographic analysis of the human histone deacetylase sirtuin 1
Reference:
Holbourn, K. P., Lloyd, M. D., Thompson, A. S., Threadgill, M. D. and Acharya, K. R., 2011. Cloning, purification, crystallization and preliminary crystallographic analysis of the human histone deacetylase sirtuin 1. Acta Crystallographica Section F-Structural Biology and Crystallization Communications, 67 (4), pp. 461-463.
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Official URL:
http://dx.doi.org/10.1107/s1744309111003277
Abstract
Human sirtuin 1 is a member of the histone deacetylase family and is involved in cellular aging, tumourigenesis and cellular metabolism. Recombinant sirtuin 1 comprising residues 140-747 was crystallized using the hanging-drop vapour-diffusion method. The crystal diffracted to 3.4 A resolution and belonged to space group P622, with unit-cell parameters a = b = 203.1, c = 625.3 A, and is estimated to contain between six and 12 molecules per asymmetric unit.
Details
| Item Type | Articles |
| Creators | Holbourn, K. P., Lloyd, M. D., Thompson, A. S., Threadgill, M. D. and Acharya, K. R. |
| DOI | 10.1107/s1744309111003277 |
| Uncontrolled Keywords | sirtuin 1, histone deacetylases |
| Departments | Faculty of Science > Biology & Biochemistry Faculty of Science > Pharmacy & Pharmacology |
| Refereed | Yes |
| Status | Published |
| ID Code | 23825 |
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