Research

Cloning and characterisation of a gene encoding a cuticle-degrading protease from the insect pathogenic fungus Metarhizium anisopliae


Reference:

Smithson, S., Paterson, I. C., Bailey, A. M., Screen, S. E., Hunt, B. A., Cobb, B. D., Cooper, R. M., Charnley, A. K. and Clarkson, J. M., 1995. Cloning and characterisation of a gene encoding a cuticle-degrading protease from the insect pathogenic fungus Metarhizium anisopliae. Gene, 166 (1), pp. 161-165.

Related documents:

This repository does not currently have the full-text of this item.
You may be able to access a copy if URLs are provided below.

Official URL:

http://dx.doi.org/10.1016/0378-1119(95)00609-3

Abstract

Metarhizium anisophilae (Ma) secretes a range of proteases when grown in vitro on insect cuticle. A trypsin-like serine protease, PR2, was purified from culture filtrates by anion exchange chromatography and the N-terminal sequence determined. Using oligodeoxyribonucleotide probes based on this sequence and that of the highly conserved trypsin active site, a gene was isolated from a λEMBL3 genomic library of Ma isolate ME1. Sequencing of the gene and RT-PCR revealed that the gene contains two introns which are 94 and 40 bp long. The deduced protein consists of 254 amino acids, has a putative signal sequence to allow transport into the endoplasmic reticulum and probably undergoes a second proteolytic processing step at its N terminus to yield the mature enzyme. The putative mature enzyme has extensive homology with other serine proteases of the trypsin subclass and, in particular, with the trypsin characterised from Fusarium oxysporum.

Details

Item Type Articles
CreatorsSmithson, S., Paterson, I. C., Bailey, A. M., Screen, S. E., Hunt, B. A., Cobb, B. D., Cooper, R. M., Charnley, A. K. and Clarkson, J. M.
DOI10.1016/0378-1119(95)00609-3
DepartmentsFaculty of Science > Biology & Biochemistry
RefereedYes
StatusPublished
ID Code24105

Export

Actions (login required)

View Item