PCR primers for microsatellite loci in a Madagascan waterbird, the Sakalava Rail (Amaurornis olivieri)
Brede, E. G., Long, P., Zefania, S., Rabenandrasana, M., Szekely, T. and Bruford, M., 2010. PCR primers for microsatellite loci in a Madagascan waterbird, the Sakalava Rail (Amaurornis olivieri). Conservation Genetics Resources, 2, pp. 273-277.
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We isolated 76 microsatellite loci from the Sakalava rail (Amaurornis olivieri) genome. These were tested in 30 individuals from six sites (in three regions) across the species' range in Madagascar. Thirty-four of the 76 loci produced unambiguous polymorphic markers, with mean H-e = 0.544 (range 0.0678-0.885), mean H-o = 0.430 (range 0.0400-0.767) and mean N-a = 4.59 (range 2-10). In addition, we tested all primers for amplification in the Wood rail, Canirallus kioloides (N = 3) and the Bemaraha rail, Canirallus sp. nov (N = 3). Twelve of the loci produced successful amplification with eight loci showing polymorphism.
|Creators||Brede, E. G., Long, P., Zefania, S., Rabenandrasana, M., Szekely, T. and Bruford, M.|
|Uncontrolled Keywords||microsatellite, waterbird, rail, endangered, primer, madagascar, amaurornis olivieri canirallus kioloides|
|Departments||Faculty of Science > Biology & Biochemistry|
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