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Microinjection of medaka embryos for use as a model genetic organism


Reference:

Porazinski, S. R., Wang, H. and Furutani-Seiki, M., 2010. Microinjection of medaka embryos for use as a model genetic organism. Journal of Visualized Experiments, 2010 (46), e1937.

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Official URL:

http://dx.doi.org/10.3791/1937

Abstract

In this video, we demonstrate the technique of microinjection into one-cell stage medaka embryos. Medaka is a small egg-laying freshwater fish that allows both genetic and embryological analyses and is one of the vertebrate model organisms in which genome-wide phenotype-driven mutant screens were carried out 1, as in zebrafish and the mouse. Divergence of functional overlap of related genes between medaka and zebrafish allows identification of novel phenotypes that are unidentifiable in a single species 2, thus medaka and zebrafish are complementary for genetic dissection of vertebrate genome functions. To take advantage of medaka fish whose embryos are transparent and develop externally, microinjection is an essential technique to inject cell-tracers for labeling cells, mRNAs or anti-sense oligonucleotides for over-expressing and knocking-down genes of interest, and DNAs for making transgenic lines.

Details

Item Type Articles
CreatorsPorazinski, S. R., Wang, H. and Furutani-Seiki, M.
DOI10.3791/1937
DepartmentsFaculty of Science > Biology & Biochemistry
RefereedYes
StatusPublished
ID Code24812

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