Plasma membrane expression of GnRH receptors: regulation by antagonists in breast, prostate, and gonadotrope cell lines.

Reference:

Finch, A. R., Sedgley, K. R., Caunt, C. J. and McArdle, C. A., 2008. Plasma membrane expression of GnRH receptors: regulation by antagonists in breast, prostate, and gonadotrope cell lines. The Journal of Endocrinology, 196 (2), pp. 353-367.

Related documents:

Official URL:

http://dx.doi.org/10.1677/JOE-07-0471

Related URLs:

• http://dx.doi.org/10.1677/JOE-07-0471

Abstract

In heterologous expression systems, human GnRH receptors (hGnRHRs) are poorly expressed at the cell surface and this may reflect inefficient exit from the endoplasmic reticulum. Here, we have defined the proportion of GnRHRs at the cell surface using a novel assay based on adenoviral transduction with epitope-tagged GnRHRs followed by staining and semi-automated imaging. We find that in MCF7 (breast cancer) cells, the proportional cell surface expression (PCSE) of hGnRHRs is remarkably low (

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