Zhou, J., Tun, T. N., Hong, S.-H., Mercer-Chalmers, J. D., Laabei, M., Young, A. E. R. and Jenkins, A. T. A., 2011. Development of a prototype wound dressing technology which can detect and report colonization by pathogenic bacteria. Biosensors and Bioelectronics, 30 (1), pp. 67-72.
A new methodology for detecting the microbiological state of a wound dressing in terms of its colonization with pathogenic bacteria such as Staphylococcus aureus or Pseudomonas aeruginosa has been developed. Here we report how stabilized lipid vesicles containing self-quenched carboxyfluorescein dye are sensitive to lysis only by toxins/virulence factors from P. aeruginosa and S. aureus but not by a non-toxic Escherichia coli species. The development of the stabilized vesicles is discussed and their response to detergent (triton), bacterial toxin (α-hemolysin) and lipases (phospholipase A2). Finally, fabrics with stabilized vesicles attached via plasma deposited maleic anhydride coupling are shown visibly responding to S. aureus (MSSA 476) and P. aeruginosa (PAO1) but not E. coli DH5α in a prototype dressing.
|Item Type ||Articles|
|Creators||Zhou, J., Tun, T. N., Hong, S.-H., Mercer-Chalmers, J. D., Laabei, M., Young, A. E. R. and Jenkins, A. T. A.|
|Departments||Faculty of Science > Chemistry|
Faculty of Science > Biology & Biochemistry
|Publisher Statement||Jenkins_Biosensors-Bioelectronics_2011.pdf: NOTICE: this is the author’s version of a work that was accepted for publication in Biosensors and Bioelectronics. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biosensors & Bioelectronics vol 30 issue 1 2011 DOI 10.1016/j.bios.2011.08.028|
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