Research

Protection against ultraviolet A-induced oxidative damage in normal human epidermal keratinocytes under post-menopausal conditions by an ultraviolet A-activated caged-iron chelator: a pilot study


Reference:

Pelle, E., Jian, J. L., Declercq, L., Dong, K., Yang, Q., Pourzand, C., Maes, D., Pernodet, N., Yarosh, D. B. and Huang, X., 2011. Protection against ultraviolet A-induced oxidative damage in normal human epidermal keratinocytes under post-menopausal conditions by an ultraviolet A-activated caged-iron chelator: a pilot study. Photodermatology Photoimmunology & Photomedicine, 27 (5), pp. 231-235.

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Official URL:

http://dx.doi.org/10.1111/j.1600-0781.2011.00604.x

Abstract

Background/purpose: Human skin is constantly exposed to ultraviolet A (UVA), which can generate reactive oxygen species and cause iron release from ferritin, leading to oxidative damage in biomolecules. This is particularly true in post-menopausal skin due to an increase in iron as a result of menopause. As iron is generally released through desquamation, the skin becomes a main portal for the release of excess iron in this age group. In the present study, we examined a strategy for controlling UVA- and iron-induced oxidative stress in skin using a keratinocyte post-menopausal cellular model system. Methods: Keratinocytes that had been cultured under normal or high-iron, low-estrogen conditions were treated with (2-nitrophenyl) ethyl pyridoxal isonicotinoyl hydrazone (2-PNE-PIH). 2-PNE-PIH is a caged-iron chelator that does not normally bind iron but can be activated by UVA radiation to bind iron. Following incubation with 2-PNE-PIH, the cells were exposed to 5 J/cm(2) UVA and then measured for changes in lipid peroxidation and ferritin levels. Results: 2-PNE-PIH protected keratinocytes against UVA-induced lipid peroxidation and ferritin depletion. Further, 2-PNE-PIH was neither cytotoxic nor did it alter iron metabolism. Conclusion: 2-PNE-PIH may be a useful deterrent against UVA-induced oxidative stress in postmenopausal women.

Details

Item Type Articles
CreatorsPelle, E., Jian, J. L., Declercq, L., Dong, K., Yang, Q., Pourzand, C., Maes, D., Pernodet, N., Yarosh, D. B. and Huang, X.
DOI10.1111/j.1600-0781.2011.00604.x
Uncontrolled Keywordskeratinocytes, chelator, uva, iron, post-menopausal
DepartmentsFaculty of Science > Pharmacy & Pharmacology
RefereedYes
StatusPublished
ID Code26768

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