Protection against ultraviolet A-induced oxidative damage in normal human epidermal keratinocytes under post-menopausal conditions by an ultraviolet A-activated caged-iron chelator: a pilot study
Pelle, E., Jian, J. L., Declercq, L., Dong, K., Yang, Q., Pourzand, C., Maes, D., Pernodet, N., Yarosh, D. B. and Huang, X., 2011. Protection against ultraviolet A-induced oxidative damage in normal human epidermal keratinocytes under post-menopausal conditions by an ultraviolet A-activated caged-iron chelator: a pilot study. Photodermatology Photoimmunology & Photomedicine, 27 (5), pp. 231-235.
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Background/purpose: Human skin is constantly exposed to ultraviolet A (UVA), which can generate reactive oxygen species and cause iron release from ferritin, leading to oxidative damage in biomolecules. This is particularly true in post-menopausal skin due to an increase in iron as a result of menopause. As iron is generally released through desquamation, the skin becomes a main portal for the release of excess iron in this age group. In the present study, we examined a strategy for controlling UVA- and iron-induced oxidative stress in skin using a keratinocyte post-menopausal cellular model system. Methods: Keratinocytes that had been cultured under normal or high-iron, low-estrogen conditions were treated with (2-nitrophenyl) ethyl pyridoxal isonicotinoyl hydrazone (2-PNE-PIH). 2-PNE-PIH is a caged-iron chelator that does not normally bind iron but can be activated by UVA radiation to bind iron. Following incubation with 2-PNE-PIH, the cells were exposed to 5 J/cm(2) UVA and then measured for changes in lipid peroxidation and ferritin levels. Results: 2-PNE-PIH protected keratinocytes against UVA-induced lipid peroxidation and ferritin depletion. Further, 2-PNE-PIH was neither cytotoxic nor did it alter iron metabolism. Conclusion: 2-PNE-PIH may be a useful deterrent against UVA-induced oxidative stress in postmenopausal women.
|Creators||Pelle, E., Jian, J. L., Declercq, L., Dong, K., Yang, Q., Pourzand, C., Maes, D., Pernodet, N., Yarosh, D. B. and Huang, X.|
|Uncontrolled Keywords||keratinocytes, chelator, uva, iron, post-menopausal|
|Departments||Faculty of Science > Pharmacy & Pharmacology|
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