Development of an amperometric, screen-printed, single-enzyme phosphate ion biosensor and its application to the analysis of biomedical and environmental samples
Gilbert, L., Jenkins, A. T. A., Browning, S. and Hart, J. P., 2011. Development of an amperometric, screen-printed, single-enzyme phosphate ion biosensor and its application to the analysis of biomedical and environmental samples. Sensors and Actuators B-Chemical, 160 (1), pp. 1322-1327.
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An amperometric phosphate biosensor, based on a cobalt phthalocyanine screen-printed carbon electrode (CoPC-SPCE) is described. The immobilisation of the enzyme pyruvate oxidase (PyOd) was investigated using pre-formed cellulose acetate/cellulose nitrate membranes, of different pore sizes, and the cross-linking agent, glutaraldehyde (GLA). The latter method was found to be superior in terms of performance characteristics and also ease of fabrication. A linear range of 2.5-130 M and limit of detection of 2 M was obtained under optimal conditions. The biosensor also exhibited an excellent response time of just 13 s 1, n = 3. The biosensor was successfully applied to the measurement of phosphate in pond water samples; the mean recovery of spiked water samples was 103.2%, n = 3. The original concentration of phosphate calculated in the water sample (48 M) was found to be in good agreement with that found using a standard colourimetric method. In addition to the analysis of water samples the biosensor was also applied to the analysis of human urine, with only a simple dilution of the sample, directly into the electrochemical cell, required for analysis. The precision of the biosensors, obtained during the urine analysis was 6.4%, n = 6.
|Creators||Gilbert, L., Jenkins, A. T. A., Browning, S. and Hart, J. P.|
|Departments||Faculty of Science > Chemistry|
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