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Heterologous Production of Functional Forms of Rhizopus oryzae Lipase in Escherichia coli


Reference:

Di Lorenzo, M., Hidalgo, A., Haas, M. and Bornscheuer, U. T., 2005. Heterologous Production of Functional Forms of Rhizopus oryzae Lipase in Escherichia coli. Applied and Environmental Microbiology, 71 (12), pp. 8974-8977.

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Official URL:

http://dx.doi.org/10.1128/AEM.71.12.8974-8977.2005

Abstract

To date, expression of the lipase from Rhizopus oryzae (ROL) in Escherichia coli always led to the formation of inclusion bodies and inactive protein. However, the production of active ROL and its precursor ProROL in soluble form was achieved when E. coli Origami(DE3) and pET-11d were used as expression systems.

Details

Item Type Articles
CreatorsDi Lorenzo, M., Hidalgo, A., Haas, M. and Bornscheuer, U. T.
DOI10.1128/AEM.71.12.8974-8977.2005
DepartmentsFaculty of Engineering & Design > Chemical Engineering
RefereedYes
StatusPublished
ID Code27198

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