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Isolation, identification and quantitation of hydroxycinnamic acid conjugates, potential platform chemicals, in the leaves and stems of Miscanthus x giganteus using LC-ESI-MS(n)


Reference:

Parveen, I., Threadgill, M. D., Hauck, B., Donnison, I. and Winters, A., 2011. Isolation, identification and quantitation of hydroxycinnamic acid conjugates, potential platform chemicals, in the leaves and stems of Miscanthus x giganteus using LC-ESI-MS(n). Phytochemistry, 72 (18), pp. 2376-2384.

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    Official URL:

    http://dx.doi.org/10.1016/j.phytochem.2011.08.015

    Abstract

    Miscanthus x giganteus is a source of platform chemicals and bioethanol through fermentation. Cinnamates in leaves and stems were analysed by LC-ESI-MS(n). Free phenols were extracted and separated chromatographically. More than 20 hydroxycinnamates were identified by UV and LC-ESI-MS(n). Comparative LC-MS studies on the leaf extract showed isomers of O-caffeoylquinic acid (3-CQA, 4-CQA and 5-CQA), O-feruloylquinic acid (3-FQA, 4-FQA and 5-FQA) and para-coumaroylquinic acid (3-pCoQA and 5-pCoQA). Excepting 3-pCoQA, all were also detected in stem. 5-CQA dominated in leaf: a mandelonitrile-caffeoylquinic acid dominated in stem. Three minor leaf components were distinguished by fragmentation patterns in a targetted MS2 experiment as dicaffeoylquinic acid isomers. Others (M(r) 516) were tentatively identified as hexosylcaffeoyl-quinates. Three positional isomers of O-caffeoylshikimic acid were minor components. p-Hydroxybenzaldehyde was also a major component in stem. This is the first report of the hydroxycinnamic acid profile of leaves and stems of M. x giganteus.

    Details

    Item Type Articles
    CreatorsParveen, I., Threadgill, M. D., Hauck, B., Donnison, I. and Winters, A.
    DOI10.1016/j.phytochem.2011.08.015
    Uncontrolled Keywordslc-esi-ms(n), high-value chemicals, hydroxycinnamic esters, miscanthus x giganteus leaf and stem tissue, uv
    DepartmentsFaculty of Science > Pharmacy & Pharmacology
    Publisher StatementThreadgill_Phytochemistry_2011_72_18_2376.pdf: NOTICE: this is the author’s version of a work that was accepted for publication in Phytochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Phytochemistry, vol 72, Issue 18, 2012, DOI 10.1016/j.phytochem.2011.08.015; Threadgill_Phytochemistry_2011_72_18_2376.doc: NOTICE: this is the author’s version of a work that was accepted for publication in Phytochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Phytochemistry, vol 72, Issue 18, 2012, DOI 10.1016/j.phytochem.2011.08.015
    RefereedYes
    StatusPublished
    ID Code27989

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