A novel role for P2X7 receptor signalling in the survival of mouse embryonic stem cells
Thompson, B. A. N., Storm, M. P., Hewinson, J., Hogg, S., Welham, M. J. and Mackenzie, A. B., 2012. A novel role for P2X7 receptor signalling in the survival of mouse embryonic stem cells. Cellular Signalling, 24 (3), pp. 770-778.
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The growth of a pluripotent embryonic stem (ES) cell population is dependent on cell survival, proliferation and self-renewal. The nucleotide ATP represents an important extracellular signalling molecule that regulates the survival of differentiated cells, however, its role is largely undefined in embryonic stem cells. Here we report a role for ATP-gated P2X7 receptors in ES cell survival. The functional expression of P2X7 receptors in undifferentiated mouse ES cells is demonstrated using a selective P2X7 antagonist and small interfering RNA knockdown of these receptors. Our data illustrate a key role for the P2X7 receptor as an essential pro-survival signal required for optimal ES cell colony growth in the presence of leukemia inhibitor factor (LIF). However, chronic exposure to exogenous ATP leads to rapid P2X7-dependent cell death via necrosis. Together, these data demonstrate a novel role for P2X7 receptors in regulation of ES cell behaviour where they can mediate either a pro-survival or pro-death signal depending on the mode of activation.
|Creators||Thompson, B. A. N., Storm, M. P., Hewinson, J., Hogg, S., Welham, M. J. and Mackenzie, A. B.|
|Departments||Faculty of Science > Pharmacy & Pharmacology|
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