Research

The influence of degree-of-branching and molecular mass on the interaction between dextran and Concanavalin A in hydrogel preparations intended for insulin release


Reference:

Benzeval, I., Bowyer, A. and Hubble, J., 2012. The influence of degree-of-branching and molecular mass on the interaction between dextran and Concanavalin A in hydrogel preparations intended for insulin release. European Journal of Pharmaceutics and Biopharmaceutics, 80 (1), pp. 143-148.

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    Official URL:

    http://dx.doi.org/10.1016/j.ejpb.2011.08.009

    Abstract

    The interactions of a number of commercially available dextran preparations with the lectin Concanavalin A (ConA) have been investigated. Dextrans over the molecular mass range 6 x 10(3)-2 x 10(6) g mol(-1) were initially characterised in terms of their branching and hence terminal ligand density, using NMR. This showed a range of branching ratios between 3% and 5%, but no clear correlation with molecular mass. The bio-specific interaction of these materials with ConA was investigated using microcalorimetry. The data obtained were interpreted using a number of possible binding models reflecting the known structure of both dextran and the lectin. The results of this analysis suggest that the interaction is most appropriately described in terms of a two-site model. This offers the best compromise for the observed relationship between data and model predictions and the number of parameters used based on the chi-squared values obtained from a nonlinear least-squares fitting procedure. A two-site model is also supported by analysis of the respective sizes of the dextrans and the ConA tetramer. Using this model, the relationship between association constants, binding energy and molecular mass was determined.

    Details

    Item Type Articles
    CreatorsBenzeval, I., Bowyer, A. and Hubble, J.
    DOI10.1016/j.ejpb.2011.08.009
    DepartmentsFaculty of Engineering & Design > Chemical Engineering
    Faculty of Engineering & Design > Mechanical Engineering
    Publisher StatementBenzeval_EJPB_2012_80_1_143.pdf: NOTICE: this is the author’s version of a work that was accepted for publication in European Journal of Pharmaceutics and Biopharmaceutics. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in European Journal of Pharmaceutics and Biopharmaceutics, vol 80, issue 1, 2012, DOI 10.1016/j.ejpb.2011.08.009; Benzeval_EJPB_2012_80_1_143.doc: NOTICE: this is the author’s version of a work that was accepted for publication in European Journal of Pharmaceutics and Biopharmaceutics. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in European Journal of Pharmaceutics and Biopharmaceutics, vol 80, issue 1, 2012, DOI 10.1016/j.ejpb.2011.08.009
    RefereedYes
    StatusPublished
    ID Code28661

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