Research

A novel transgenic line using the Cre-lox system to allow permanent lineage-labeling of the zebrafish neural crest


Reference:

Rodrigues, F. S. L. M., Doughton, G., Yang, B. and Kelsh, R. N., 2012. A novel transgenic line using the Cre-lox system to allow permanent lineage-labeling of the zebrafish neural crest. Genesis, 50 (10), pp. 750-757.

Related documents:

This repository does not currently have the full-text of this item.
You may be able to access a copy if URLs are provided below. (Contact Author)

Official URL:

http://dx.doi.org/10.1002/dvg.22033

Abstract

Accurate lineage tracing is crucial to understanding of developmental and stem cell biology, but is particularly challenging for transient and highly dispersive cell-types like the neural crest (NC). The authors report in this article a new zebrafish transgenic line Tg(-4725sox10:Cre) ba74. This line expresses Cre under the control of a well-characterized portion of the sox10 promoter and, by crossing to a floxed-reporter line, the authors show in this article that expression in this line is consistent with those described for GFP reporter lines using the same promoter. Reporter expression is readily detected in patterns consistent with the early expression domains. Thus, the authors see all major groups (pigment, neural, and skeletal) of NC-derived cell-types, as well as cell-types derived from the known non-NC sites of sox10 expression, including otic epithelium and oligodendrocytes. This line provides an invaluable tool for the further study of zebrafish NC development and NC-derived stem cells as well as that of the otic vesicle and oligodendrocytes.

Details

Item Type Articles
CreatorsRodrigues, F. S. L. M., Doughton, G., Yang, B. and Kelsh, R. N.
DOI10.1002/dvg.22033
DepartmentsFaculty of Science > Biology & Biochemistry
Research CentresCentre for Regenerative Medicine
RefereedYes
StatusPublished
ID Code30298

Export

Actions (login required)

View Item