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Involvement of adenosine in ethanol-induced changes in G-protein expression in NG108-15 cells?


Reference:

Williams, R. J., Veale, M. A. and Kelly, E., 1993. Involvement of adenosine in ethanol-induced changes in G-protein expression in NG108-15 cells? Alcohol and Alcoholism: Supplement, 2, pp. 431-435.

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Abstract

Previous work has suggested that adenosine may be involved in ethanol-induced heterologous desensitization of adenylate cyclase in NG108-15 cells. It was proposed that chronic ethanol causes adenosine to accumulate extracellularly, activating adenosine A2 receptors and so leading to a reduction in Gs alpha mRNA and Gs alpha protein (Nagy et al., 1989). In this study we further investigated the effect of chronic ethanol on G-protein expression in NG108-15 cells. Pretreatment of NG108-15 cells with ethanol (200 mM, 48h) reduced membrane levels of Gs alpha and Gi alpha but increased Go alpha expression. The effects of ethanol on alpha-subunit expression were not reversed by adenosine deaminase and could not be mimicked by the adenosine agonist 5'- (n-ethyl)-carboxamidoadenosine (NECA). Chronic ethanol pretreatment did not appear to reduce the levels of Gs alpha or Gi alpha 2 mRNA. This same ethanol pretreatment reduced cell proliferation and increased differentiation without altering cell viability. Adenosine deaminase did not reverse any of these effects. These results indicate that ethanol differentially regulates G-protein alpha-subunit expression and induces morphological alterations in these cells independently of extracellular adenosine.

Details

Item Type Articles
CreatorsWilliams, R. J., Veale, M. A. and Kelly, E.
Related URLs
URLURL Type
http://www.ncbi.nlm.nih.gov/pubmed/7748334PubMedCentral
DepartmentsFaculty of Science > Biology & Biochemistry
RefereedYes
StatusPublished
ID Code30582

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