Bagnall, J.P., Evans, S.E., Wort, M.E., Lubben, A. and Kasprzyk-hordern, B., 2012. Using chiral liquid chromatography quadrupole time-of-flight mass spectrometry for the analysis of pharmaceuticals and illicit drugs in surface and wastewater at the enantiomeric level. Journal of Chromatography A, 1249, pp. 115-129.
This paper presents and compares for the first time two chiral LC-QTOF-MS methodologies (utilising CBH and Chirobiotic V columns with cellobiohydrolase and vancomycin as chiral selectors) for the quantification of amphetamine. methamphetamine, MDA (methylenedioxyamphetamine), MDMA (methylenedioxymethamphetamine), propranolol. atenolol, metoprolol, fluoxetine and venlafaxine in river water and sewage effluent. The lowest MDLs (0.3-5.0 ng L-1 and 1.3-15.1 ng L-1 for river water and sewage effluent respectively) were observed using the chiral column Chirobiotic V. This is with the exception of methamphetamine and MDMA which had lower MDLs using the CBH column. However, the CBH column resulted in better resolution of enantiomers (R-s = 2.5 for amphetamine compared with R-s = 1.2 with Chirobiotic V). Method recovery rates were typically >80% for both methodologies. Pharmaceuticals and illicit drugs detected and quantified in environmental samples were successfully identified using MS/MS confirmation. In sewage effluent, the total beta-blocker concentrations of propranolol, atenolol and metoprolol were on average 77.0, 1091.0 and 3.6 ng L-1 thus having EFs (Enantiomeric Fractions) of 0.43, 0.55 and 0.54 respectively. In river water, total propranolol and atenolol was quantified on average at <10.0 ng L-1. Differences in EF between sewage and river water matrices were evident: venlafaxine was observed with respective EF of 0.43 +/- 0.02 and 0.58 +/- 0.02.
|Item Type ||Articles|
|Creators||Bagnall, J.P., Evans, S.E., Wort, M.E., Lubben, A. and Kasprzyk-hordern, B.|
|Departments||Faculty of Science|
Faculty of Science > Chemistry
|Research Centres||Centre for Sustainable Chemical Technologies|
|Publisher Statement||JPB_et_al_J_Chromatogr_2012_1249_115.pdf: NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Chromatography A. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Chromatography A, vol 1249, 2012, DOI 10.1016/j.chroma.2012.06.012|
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