Application of pheB as a reporter gene for Geobacillus spp, enabling qualitative colony screening and quantitative analysis of promoter strength

Reference:

Bartosiak-Jentys, J., Eley, K. and Leak, D. J., 2012. Application of pheB as a reporter gene for Geobacillus spp, enabling qualitative colony screening and quantitative analysis of promoter strength. Applied and Environmental Microbiology, 78 (16), pp. 5945-5947.

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Official URL:

http://dx.doi.org/10.1128/AEM.07944-11

Abstract

The pheB gene from Geobacillus stearothermophilus DSM6285 has been exploited as a reporter gene for Geobacillus spp. The gene product, catechol 2,3dioxygenase (C23O), catalyses the formation of 2-hydroxymuconic semialdehyde which can be readily assayed. The reporter was used to examine expression from the ldh promoter associated with fermentative metabolism.

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