McHugh, P. C., Wright, J. A., Williams, R. J. and Brown, D. R., 2012. Prion protein expression alters APP cleavage without interaction with BACE-1. Neurochemistry International, 61 (5), pp. 672-680.
The prion protein (PrP) and the beta-site amyloid precursor protein (APP) cleaving enzyme 1 (BACE-1) are both copper binding proteins, but are associated with two separate neurodegenerative diseases. The role of BACE-1 in the formation of beta-amyloid has made it a major target in attempts to reduce the formation of beta-amyloid in Alzheimer's diseases. However, the suggestion that PrP, normally associated with prion diseases, binds to BACE-1 and reduces its activity has led to the suggestion that the study of this interaction could be of considerable importance to Alzheimer's disease. We therefore undertook to investigate the possible interaction of these two proteins physically and at the level of transcription, translation and APP cleavage. Our findings suggest that mature PrP and BACE-1 do not physically interact, but that altered PrP expression results in altered BACE-1 protein expression and promoter activity. Additionally, overexpression of PrP results in increased cleavage of APP in contrast to previous datas suggesting a reduction. Our findings suggest that any relation between PrP and BACE-1 is indirect. Altered expression of PrP causes changes in the expression of many other proteins which may be as a result of altered copper metabolism.
|Item Type ||Articles|
|Creators||McHugh, P. C., Wright, J. A., Williams, R. J. and Brown, D. R.|
|Departments||Faculty of Science > Biology & Biochemistry|
|Research Centres||Centre for Regenerative Medicine|
|Publisher Statement||Brown_Neurchem_Int_2012_61_5_672.pdf: NOTICE: this is the author’s version of a work that was accepted for publication in Neurochemistry International. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Neurochemistry International, 61(5), 2012, DOI 10.1016/j.neuint.2012.07.002|
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