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In vivo imaging of tight junctions using claudin-EGFP transgenic medaka


Reference:

Miyamoto, T., Furuse, M. and Furutani-Seiki, M., 2011. In vivo imaging of tight junctions using claudin-EGFP transgenic medaka. In: Kursad, T., ed. Claudins. Vol. 762. New York: Humana Press, pp. 171-178.

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Official URL:

http://dx.doi.org/10.1007/978-1-61779-185-7_12

Abstract

Tight junctions (TJs) function as a physiological barrier between epithelial and endothelial sheets by restricting the diffusion of fluid through the intercellular space. Recent morphological studies associated with TJs have revealed that the TJ is a dynamic rather than a static structure; indeed, several in vitro studies indicate that proper TJ function requires dynamic TJ behavior. Direct observation of the dynamic behavior of TJs is necessary to understand the essential roles of TJs in physiological contexts, such as during embryogenesis and metastasis. Here we describe a protocol for the generation of transgenic medaka (Oryzias latipes) that express claudin–EGFP. This fluorescent fusion protein enables real-time imaging of TJs in the living embryo. Claudin–EGFP transgenic medaka will be a useful tool to screen for mutations and for small molecules affecting cell–cell adhesion.

Details

Item Type Book Sections
CreatorsMiyamoto, T., Furuse, M. and Furutani-Seiki, M.
EditorsKursad, T.
DOI10.1007/978-1-61779-185-7_12
DepartmentsFaculty of Science > Biology & Biochemistry
Research CentresCentre for Regenerative Medicine
RefereedNo
StatusPublished
ID Code32737

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