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Analysis of protein glycation using fluorescent phenylboronate gel electrophoresis


Reference:

Pereira Morais, M. P., Marshall, D., Flower, S. E., Caunt, C. J., James, T. D., Williams, R. J., Waterfield, N. R. and Van Den Elsen, J. M. H., 2013. Analysis of protein glycation using fluorescent phenylboronate gel electrophoresis. Scientific Reports, 3, 1437.

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Abstract

Glycated proteins are important biomarkers for age-related disorders, however their analysis is challenging because of the complexity of the protein-carbohydrate adducts. Here we report a method that enables the detection and identification of individual glycated proteins in complex samples using fluorescent boronic acids in gel electrophoresis. Using this method we identified glycated proteins in human serum, insect hemolymph and mouse brain homogenates, confirming this technique as a powerful proteomics tool that can be used for the identification of potential disease biomarkers.

Details

Item Type Articles
CreatorsPereira Morais, M. P., Marshall, D., Flower, S. E., Caunt, C. J., James, T. D., Williams, R. J., Waterfield, N. R. and Van Den Elsen, J. M. H.
DOI10.1038/srep01437
Related URLs
URLURL Type
http://www.scopus.com/inward/record.url?scp=84875780786&partnerID=8YFLogxKUNSPECIFIED
http://dx.doi.org/10.1038/srep01437Free Full-text
DepartmentsFaculty of Science > Biology & Biochemistry
Faculty of Science > Chemistry
Research CentresCentre for Sustainable Chemical Technologies
Centre for Regenerative Medicine
RefereedYes
StatusPublished
ID Code34357

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