Research

Studying the cytolytic activity of gas plasma with self-signalling phospholipid vesicles dispersed within a gelatin matrix


Reference:

Marshall, S., Jenkins, A. T. A., Al-Bataineh, S. A., Short, R. D., Hong, S.-H., Thet, N. T., Oh, J.-S., Bradley, J. W. and Stili, E. J., 2013. Studying the cytolytic activity of gas plasma with self-signalling phospholipid vesicles dispersed within a gelatin matrix. Journal of Physics D: Applied Physics, 46 (18), 185401.

Related documents:

This repository does not currently have the full-text of this item.
You may be able to access a copy if URLs are provided below. (Contact Author)

Official URL:

http://dx.doi.org/10.1088/0022-3727/46/18/185401

Related URLs:

Abstract

A synthetic biological sensor was developed to monitor the interaction of plasma with soft, hydrated biological material. It comprises phospholipid vesicles in a hydrated proteinaceous environment comprising 5% (w/v) gelatin. The vesicles contained a self-quenched dye, which was activated by vesicle destruction giving a clear fluorescent switch on. The interaction of bacterial toxins with the sensor was measured in a proof of principle experiment, then the effect of atmospheric plasma jets with the sensor, was studied in order to assess the cytolytic effect of plasma jets in biological systems. When the plasma contacted the gelatin surface perpendicular to the surface, the treatment resulted in the formation of a star-shaped pattern of microchannels that radiated out from the centre of the treatment area within the gelatin matrix, and locally damaged vesicles within the microchannels at a depth of 150 µm below the gelatin surface. Plasma jets applied in parallel to the surface of the matrix resulted in the formation of a single microchannel with damage to the vesicles only evident at the walls of the channel, and a much reduced penetration depth within the gelatin. Our data show that the effects of plasma can be deep in the gelatin material and that the angle of treatment significantly influenced the nature and level of damage to the gelatin and vesicles. Potentially this gelatin model can be used to unravel the roles of different plasma species and the direct effect of whole plasma contact, from those of primary and secondary species—i.e. primary, those emanating directly from the plasma and secondary, those species created in the 'target' tissue. This type of insight could be useful in the future development of safe and effective plasma medical technologies.

Details

Item Type Articles
CreatorsMarshall, S., Jenkins, A. T. A., Al-Bataineh, S. A., Short, R. D., Hong, S.-H., Thet, N. T., Oh, J.-S., Bradley, J. W. and Stili, E. J.
DOI10.1088/0022-3727/46/18/185401
Related URLs
URLURL Type
http://www.scopus.com/inward/record.url?scp=84876543686&partnerID=8YFLogxKUNSPECIFIED
DepartmentsFaculty of Science > Chemistry
Research CentresCentre for Sustainable Chemical Technologies
RefereedYes
StatusPublished
ID Code34739

Export

Actions (login required)

View Item