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A Golgi and tonoplast localized S-acyl transferase is involved in cell expansion, cell division, vascular patterning and fertility in Arabidopsis


Reference:

Qi, B., Doughty, J. and Hooley, R., 2013. A Golgi and tonoplast localized S-acyl transferase is involved in cell expansion, cell division, vascular patterning and fertility in Arabidopsis. New Phytologist, 200 (2), pp. 444-456.

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    Official URL:

    http://dx.doi.org/10.1111/nph.12385

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    Abstract

    Summary: S-acylation of eukaryotic proteins is the reversible attachment of palmitic or stearic acid to cysteine residues, catalysed by protein S-acyl transferases that share an Asp-His-His-Cys (DHHC) motif. Previous evidence suggests that in Arabidopsis S-acylation is involved in the control of cell size, polarity and the growth of pollen tubes and root hairs. Using a combination of yeast genetics, biochemistry, cell biology and loss of function genetics the roles of a member of the protein S-acyl transferase PAT family, AtPAT10 (At3g51390), have been explored. In keeping with its role as a PAT, AtPAT10 auto-S-acylates, and partially complements the yeast akr1 PAT mutant, and this requires Cys192 of the DHHC motif. In Arabidopsis AtPAT10 is localized in the Golgi stack, trans-Golgi network/early endosome and tonoplast. Loss-of-function mutants have a pleiotropic phenotype involving cell expansion and division, vascular patterning, and fertility that is rescued by wild-type AtPAT10 but not by catalytically inactive AtPAT10C192A. This supports the hypothesis that AtPAT10 is functionally independent of the other Arabidopsis PATs. Our findings demonstrate a growing importance of protein S-acylation in plants, and reveal a Golgi and tonoplast located S-acylation mechanism that affects a range of events during growth and development in Arabidopsis.

    Details

    Item Type Articles
    CreatorsQi, B., Doughty, J. and Hooley, R.
    DOI10.1111/nph.12385
    Related URLs
    URLURL Type
    http://www.scopus.com/inward/record.url?scp=84884413786&partnerID=8YFLogxKUNSPECIFIED
    Uncontrolled Keywordsarabidopsis,dhhc-pat,vascular patterning,trans-golgi network,tonoplast,protein s-acylation,dwarfism
    DepartmentsFaculty of Science > Biology & Biochemistry
    Publisher Statementnph12385.pdf: © 2013 The Authors. New Phytologist © 2013 New Phytologist Trust This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. The definitive version is available at http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1469-8137;Qi_2013_NewPhytologist.pdf: © 2013 The Authors New Phytologist © 2013 New Phytologist Trust This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
    RefereedYes
    StatusPublished
    ID Code35815

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