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Glucose oxidase directly immobilized onto highly porous gold electrodes for sensing and fuel cell applications


Reference:

Du Toit, H. and Di Lorenzo, M., 2014. Glucose oxidase directly immobilized onto highly porous gold electrodes for sensing and fuel cell applications. Electrochimica Acta, 138, pp. 86-92.

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    Official URL:

    http://dx.doi.org/10.1016/j.electacta.2014.06.074

    Abstract

    The successful implementation of redox-enzyme electrodes in biosensors and enzymatic biofuel cells has been the subject of extensive research. For high sensitivity and high energy-conversion efficiency, the effective electron transfer at the protein-electrode interface has a key role. This is difficult to achieve in the case of glucose oxidase, due to the fact that for this enzyme the redox centre is buried inside the structure, far from any feasible electrode binding sites. This study reports, a simple and rapid methodology for the direct immobilisation of glucose oxidase into highly porous gold electrodes. When the resulting electrode was tested as glucose sensor, a Michaelis-Menten kinetic trend was observed, with a detection limit of 25 μM. The bioelectrode sensitivity, calculated against the superficial surface area of the bioelectrode, was of 22.7± 0.1 μA mM-1cm-2. This glucose oxidase electrode was also tested as an anode in a glucose/O2 enzymatic biofuel cell, leading to a peak power density of 6 µW cm-2 at a potential of 0.2 V.

    Details

    Item Type Articles
    CreatorsDu Toit, H.and Di Lorenzo, M.
    DOI10.1016/j.electacta.2014.06.074
    DepartmentsFaculty of Engineering & Design > Chemical Engineering
    Research CentresCentre for Sustainable Chemical Technologies
    RefereedYes
    StatusPublished
    ID Code39796

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