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Incorporation of Sed1p into the cell wall of Saccharomyces cerevisiae involves KRE6


Reference:

Bowen, S. and Wheals, A. E., 2004. Incorporation of Sed1p into the cell wall of Saccharomyces cerevisiae involves KRE6. FEMS Yeast Research, 4 (7), pp. 731-735.

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Official URL:

http://dx.doi.org/10.1016/j.femsyr.2004.02.006

Abstract

KRE6 (YPR159W) encodes a Golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. A functional Kre6p is necessary for cell wall protein accumulation in response to changing metabolic conditions. The product of the SEDI (YDR077W) gene is a stress-induced GPI-cell wall protein. Successful incorporation of HA-tagged Sed1p into the cell wall involves KRE6. The double-mutant sed1 kre6 has a reduced growth rate, increased flocculation and increased sensitivity to Zymolyase. A similar phenotype is found in mutants defective in glycosyl-phosphatidyl-insositol (GPI) anchor assembly. These findings support the theory that Kre6p could function as a transglucosylase that allows the incorporation of proteins with a GPI anchor into the cell wall.

Details

Item Type Articles
CreatorsBowen, S.and Wheals, A. E.
DOI10.1016/j.femsyr.2004.02.006
DepartmentsFaculty of Science > Biology & Biochemistry
RefereedYes
StatusPublished
ID Code4009

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