Incorporation of Sed1p into the cell wall of Saccharomyces cerevisiae involves KRE6
Bowen, S. and Wheals, A. E., 2004. Incorporation of Sed1p into the cell wall of Saccharomyces cerevisiae involves KRE6. FEMS Yeast Research, 4 (7), pp. 731-735.
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KRE6 (YPR159W) encodes a Golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. A functional Kre6p is necessary for cell wall protein accumulation in response to changing metabolic conditions. The product of the SEDI (YDR077W) gene is a stress-induced GPI-cell wall protein. Successful incorporation of HA-tagged Sed1p into the cell wall involves KRE6. The double-mutant sed1 kre6 has a reduced growth rate, increased flocculation and increased sensitivity to Zymolyase. A similar phenotype is found in mutants defective in glycosyl-phosphatidyl-insositol (GPI) anchor assembly. These findings support the theory that Kre6p could function as a transglucosylase that allows the incorporation of proteins with a GPI anchor into the cell wall.
|Creators||Bowen, S.and Wheals, A. E.|
|Departments||Faculty of Science > Biology & Biochemistry|
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